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The FASTQ input file size for free data analysis runs is 1.5 GB. Larger FASTQ file sizes can only be processed using codes for large file sizes, which can be purchased additionally (Cat. No. 211 and 212 for QuantSeq FWD; Cat. No. 213 and 214 for QuantSeq REV). Alternatively, you can compress your FASTQ files. For more information on how to achieve this, please see: https://faqs.lexogen.com/faq/my-fastq-files-are-bigger-than-1-5-gb-what-should-