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PCR is extremely sensitive! The Polymerase Chain Reaction (PCR) amplifies minuscule amounts of DNA. Therefore, even a trace of amplified DNA (from a previous experiment) can contaminate new samples or reagents used in the assay, thus falsifying results. To learn more about PCR, see https://www.lexogen.com/rna-lexicon-rna-seq-library-preparation-molecular-biology-basics/ our RNA Lexicon chapter on RNA-Seq Library Preparation.

We recommend you have two spaces (areas) for pre- and post-PCR work!

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