QuantSeq libraries are intended for a high degree of multiplexing. Indices are introduced as standard external barcodes during the Endpoint PCR amplification step. Up to 96 i7 indices are included in the single indexing kits (Cat. No. 015).
For streamlined . For convince, our QuantSeq Kits are available with up to 384 Unique Dual Indexing (UDIUDIs), : QuantSeq FWD is available in convenient bundles including up to 384 pre-mixed Lexogen 12 nt UDIs (Cat. No. 113.96, 114.96, 129.96, 130.96, 131.96, and 115.384).
Multiplexing QuantSeq libraries with other library types in the same sequencing lane is not recommended. For further information, please see FAQ 28191 -196) and QuantSeq REV (Cat. No. 225).
Lexogen’s 12 nt UDIs are also available as stand-alone Add-on Kits (indices and PCR reagents) or Index Sets (indices only).