Input amounts between 10 ng - 1 µg of total RNA can be used for poly(A) selection prior to CORALL RNA-Seq for Blood library preparation. RNA should be free of genomic DNA and other contaminants such as salts, metal ions, and organic solvents, which can be carried over from RNA extraction. Several sources of contamination can be detected with an UV-Vis spectrophotometer. An acceptably pure RNA sample should have an A260/A280 ratio between 1.8 and 2.1. The A260/A230 ratio should also be greater than1.8.
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The RNA should have an integrity score (RIN, RQN) of 7 or higher.
NOTE: The following protocol is a short version and first time users are advised to refer to the individual User Guides of Poly(A) RNA Selection, RiboCop Human/Mouse/Rat and CORALL RNA-Seq V2 for detailed protocols.Table of Contents:
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1. Poly(A) RNA Selection
Cat. No. 157 - Poly(A) RNA Selection Kit V1.5
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