The free BlueBee Data Analysis data analysis runs included with QuantSeq FWD/REV kits are limited to FASTQ files with a maximum size of 1.5 GB. If you have larger files you have two options:
Option 1
You can compress your FASTQ files and/or sub-sample the reads to reduce the size of your FASTQ files to 1.5 GB or less.
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For windows computers download 7-zip and re-zip with quality best.
For Mac OS and Linux this can be done with the command line command: gunzip -c some_file.fastq.gz | gzip -9 > some_higher_compressed_file.fastq.gz
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To sub-sample reads:
Check out this Subsampling a fastq file with awk a command line tutorial.
Option 2
You can purchase Activation Codes data analysis codes for larger file sizes by contacting sales@lexogen.com. Here are the catalog numbers you need:
Catalog No. | Product Name |
211. |
01 | QuantSeq Data Analysis (FWD/FWD-UMI) |
, 1 run, 0 – 3 GB |
212. |
01 | QuantSeq Data Analysis (FWD/FWD-UMI) |
, 1 run, 0 – 6 GB |
213. |
01 | QuantSeq Data Analysis (REV) |
, 1 run, 0 – 3 GB |
214. |
01 | QuantSeq Data Analysis (REV) |
, 1 run, 0 – 6 GB |
Please note there are different catalog numbers for FWD or REV runs as this controls the access to the specific analysis pipeline.
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