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The NovaSeq Xp workflow enables individual lane loading on the same flow cell. Hence the multiplexing capacity can be expanded 2 times using the NovaSeq XP 2-Lane Kit or 4 times with the NovaSeq XP 4-Lane Kit. Hence, it is possible load up to 4 physically separated 384-plex UDI-indexed libraries per flow cell. For more information about the Xp Workflow, please refer to the NovaSeq™ Xp workflow Technical Note, The NovaSeq™ Xp workflow explanatory video, Yes. The NovaSeq 6000 v1.5 Reagent Kits include additional SBS chemistry cycles, which are increased from 30 to 38 compared to the previous v1.0 Reagent Kits. Thus, expanding the maximum number of supported cycles per kit to 538, 338, 238, and 138 for 500, 300, 200, and 100-cycle kits, respectively. Moreover, in the new v1.5 Kits, the Reverse Complement Workflow (B) replaces the Forward Strand Workflow (A) utilized by the v1.0 Kits. This change further impacts the number of effective SBS chemistry cycles because the Reverse Complement Workflow does not require additional seven cycles of reagents for the chemistry-only cycles at the beginning of the second Index Read. Therefore, with additional 38 effective SBS chemistry cycles, as opposed to only 23 with v1.0 Kits, one can comfortably read out 12 + 12 nt from the Lexogen UDIs. For more information please refer to the https://emea.support.illumina.com/bulletins/2016/10/how-many-cycles-of-sbs-chemistry-are-in-my-kit.html Illumina bulletin or contact Illumina Support.