The UMI sequence (6 nt) and following 4 nt spacer is read-out at the beginning of Read 1. Hence, additional nucleotides at the 5' end of each read will be taken up by the UMI + spacer. We therefore recommend a minimum read length of 75 bp (i.e., SR75) or longer (and up to SR150) , for sequencing QuantSeq FWD libraries containing UMIs.
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