The For QuantSeq REV V2 Kits (Cat. No. 225), the recommended input range is 10 1 - 500 ng of total RNA. Protocol modifications apply for input amounts below 10ng of total RNA (please see the QuantSeq REV V2 User Guide; Appendix B).
No prior poly(A) enrichment or rRNA depletion is required.
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As a starting point, we recommend performing the protocol initially with 500 ng total RNA. RNA inputs ≥200 ng are recommended to detect low abundant transcripts efficiently.]If you are limited in terms of input, we recommend using QuantSeq FWD. By choosing longer sequencing read lengths, you are also able to read into the 3' ends.