Lexogen UDI 12 nt Sets contain a unique nested feature enabling adjustable index read-out lengths (the longer the index read-out, the better the error correction). The UDIs are 12 nucleotides long, however, the superior error correction feature can also be used if only 8 or 10 nucleotides of the index are read out, if the i5 index was read out in the correct orientation see Figure 1 and also FAQ 11.
[Quality]
Figure 1 | Optional read-out lengths for Lexogen UDI 12 nt SetsIf your library yields are extremely low and insufficient for pooling, reamplification can be performed using the PCR Add-on and Reamplification Kit V2 (Cat. No. 208.96).
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