The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) is compatible with the following library prep kits:
QuantSeq 3' 3’ mRNA-Seq V2 Library Prep Kits for Illumina (FWD, Kit FWD with UDI (Cat. No. s 015, 113 – 115, 129 – 131191 - 196)
QuantSeq -Flex Targeted RNA3’ mRNA-Seq V2 Library Prep Kit V2 with FWD with UDI when used in combination with the QuantSeq-Flex First Strand Synthesis Module for Illumina V2 (Cat. No. 033) using oligo(dT) or target-specific first strand synthesis priming and random primed second strand synthesis166)
These modules can also be used for QuantSeq library preparation where UMIs are included:
Globin Block Modules for QuantSeq (Human and Pig, Cat. No. s 070, 071)
i5 Lexogen UDI 12 nt Unique Dual Indexing V2 Add-on Kits for QuantSeq / CORALL (Cat. No. 047198 - 203)
Lexogen UDI 12 nt Unique Dual Indexing Add-on Kits and Index Sets (Cat. No. s 101 - 105, 107 - 111, 120, and 156)
PCR Add-on Kit for Illumina (Cat. No. 020208)
This UMI module is not compatible with the following kits:
QuantSeq 3' mRNA-Seq Library Prep Kit REV ( for Illumina , (Cat. No. 016225)
QuantSeq -Flex First Strand Synthesis Module of Illumina V2 FFPE 3' mRNA-Seq Library Prep Kit FWD with UMIs (Cat. No. 166222-223)
QuantSeq-Flex Second Strand Synthesis Module for Illumina (Cat. No. 028)
CORALL LUTHOR HD 3' mRNA-Seq and CORALL Total RNALibrary Prep Kit for Illumina (Cat. No.204, 221)
LUTHOR HD Pool ' mRNA-Seq Library Prep Kit for Illumina (Cat. No. 205)
CORALL V2 Library Prep Kits (Cat. No. 158 – 163 177 - 182 for CORALL mRNA-Seq and V2, Cat. No. 095, 096, 117 – 119, 132 – 134, 146, 147 for CORALL 171 – 176, 183 - 186 for CORALL V2 Total RNA-Seq and Cat. No. 219-220, 233-234 for CORALL FFPE Total RNA-Seq)
Small RNA-Seq Library Prep Kit for Illumina (Cat. No. 052, 058, 135)
ATTENTION! The UMI Second Strand Synthesis Module for QuantSeq is also not compatible with, and not recommended for use with library prep protocols or kits from any other vendors.
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