RNA input amounts lower than 100 ng (for total RNA or enriched small RNA) require protocol adjustments. These adjustments include dilution of the 3' Adapter (A3), 5' Adapter , and RT-Primer (A5) and Reverse Transcription Primer (RTP) and adjusted endpoint PCR cycle numbers (see Appendix B of the User Guide).
The minimum amount of total RNA input depends on the small RNA content of the sample in question.
For samples with a small RNA content of 10 %, 1 ng total RNA may be used as the minimum input.
Increase the input material for total RNA samples with lower small RNA content.
...