The Small RNA-Seq Library Prep Kit is appropriate for sequencing on Illumina platforms (HiSeq2000, HiSeq3000, HiSeq4000, NextSeq 500 / 550, NextSeq 5502000, NovaSeq 6000, MiSeq, MiniSeq, and GAIIX).
The libraries are compatible with standard Illumina sequencing primers for TruSeq Small RNA and no custom sequencing primers are required.Current
For current loading amount recommendations are available for the following instruments:
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MiSeq / HiSeq2500 / HiSeq4000: 15 – 20 pM
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please see: Do you have recommended loading concentrations for Lexogen libraries on Illumina sequencing instruments?