OLD
LUTHOR LUTHOR HD can be used for direct RNA amplification from animal cells or protoplasts (e.g., from plants) , or with purified RNA from all organisms and viruses that possess poly(A)-tailed polyadenylated RNA species.
LUTHOR HD is not suitable for the analysis of non-polyadenylated RNA, e.g., total RNA from bacterial species.
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FACS-Sorted Cells: 1 – 100 cells per well/sample may be used.
For sorting of single cells, we recommend sorting directly into 5 µl of Cell Lysis Buffer (CLB).Cell suspensions: 1 – 100 cells/sample in a volume of 2 µl. Prepare cell suspension in 1x PBS.
Purified RNA: 10 pg – 1 ng in a volume of 4 µl.
Higher inputs are possible and require minor protocol modifications. For more information, please contact Lexogen Tech Support at support@lexogen.com.
NEW
LUTHOR HD can be used for direct RNA amplification from animal cells or protoplasts (e.g., from plants) or with purified RNA from all organisms and viruses that possess poly(A)-tailed RNA species.
LUTHOR HD is not suitable for analysis of non-polyadenylated RNA, e.g., total RNA from bacterial species.
Input ranges:
FACS-Sorted Cells: 1 – 100 cells per well/sample may be used. For sorting of single cells, we recommend sorting directly into 5 µl of Cell Lysis Buffer (CLB).Cell suspensions: 1 or more. Prepare cell suspension in 1x PBS.
Purified RNA: 10 pg – 1 ng in a volume of 3 µl.
Higher inputs are not recommended, and samples should be diluted to meet recommended input guidelines. For more information, please contact Lexogen Tech Support at support@lexogen.com.
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