The TeloPrime protocol has been validated with input amounts of 1 ng to 2 µg of total RNA input using Universal Human Reference RNA (Agilent Technologies), as well as RNA extracted from mouse liver, kidney, brain, lung, spleen, thymus, heart, and Human Brain Reference RNA. We recommend using the highest quality total RNA as input to maximize the yield and length of the amplified cDNA.
For long-read sequencing applications we recommend using higher input amounts of 1 – 2 µg of total RNA. See also Appendix A of the TeloPrime V2 User Guide for more details.
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