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Lexogen Data Analysis
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Solution
QuantSeq FWD-UMI libraries.
Starting from January 2023, to analyze data from QuantSeq FWD libraries that contain UMIs, use the activation code included in your QuantSeq FWD library prep kit and follow the instruction provided in this online FAQ for submitting your Data Analysis Request.
For further details see How can I analyze my QuantSeq data using Lexogen data analysis solutions?
For updates on the data analysis solutions provided by Lexogen, please check this Webpage: Lexogen Data Analysis Solutions | Lexogendata can be analyzed using our new web-based interactive platform, Kangooroo. QuantSeq kits are provided with a voucher code which can be used for the data analysis. If additional codes are needed, please contact sales@lexogen.com.
For further information about how to process your data, please visit our Webpage https://www.lexogen.com/lexogen-data-analysis-solutions/ and check out these online FAQs.
UMI-Tools
As an alternative to the data analysis solutions solution offered by Lexogen, we recommend utilizing the publicly available UMI-Tools package available on GitHub here. Detailed documentation can be found in the ReadTheDocs. The following command line will extract the UMI sequence from the read while removing the adjacent 4 nt TATA spacer:
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The deduplication method of UMI-Tools has been published here.
NOTE: The current implementation of this method can take some time and can consume significant memory. If you experience issues with run time or memory usage, please refer to these FAQs.
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For further information contact us at support@lexogen.com.