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Lexogen Data Analysis
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Solution
QuantSeq FWD-UMI data can be analyzed using our in-house automated Lexogen data analysis system https://lexogen-customerportal.atlassian.net/servicedesk/customer/portal/12/group/25/create/64 or our new web-based interactive platform, Kangooroo. QuantSeq kits are provided with a voucher codes code which can be used for the data analysis. If additional codes are needed, please contact sales@lexogen.com.
For further information about how to process your data, please visit our Webpage https://www.lexogen.com/lexogen-data-analysis-solutions/ and check out these online FAQs.
UMI-Tools
As an alternative to the data analysis solutions solution offered by Lexogen, we recommend utilizing the publicly available UMI-Tools package available on GitHub here. Detailed documentation can be found in the ReadTheDocs. The following command line will extract the UMI sequence from the read while removing the adjacent 4 nt TATA spacer:
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The deduplication method of UMI-Tools has been published here.
NOTE: The current implementation of this method can take some time and can consume significant memory. If you experience issues with run time or memory usage, please refer to these FAQs.
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