No, currently the QuantSeq data analysis pipelines on BlueBee are only compatible with standard QuantSeq FWD and REV. QuantSeq-Pool libraries contain the Read 1 linker sequence in the 5' part of the second strand synthesis primer, hence NGS reads are generated towards the poly(A) tail. Following demultiplexing, all further steps for trimming, mapping, UMI processing, and counting can be integrated in standard data analysis pipelines. Lexogen offers a QuantSeq-Pool data analysis pipeline, which can be found on our GitHub page. For further questions about the pipeline, please contact support@lexogen.com.
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