I see poly(T) stretches in my QuantSeq REV data and low sequencing quality. What is the reason for this?

For QuantSeq REV, it is essential to use the Custom Sequencing Primer (CSP; (provided with the QuantSeq kit) for Read 1 sequencing. Make sure that your sequencing facility is aware of this. When handing in QuantSeq REV libraries for sequencing, please include the CSP and the information on how to use the CSP. The CSP should never be mixed together with the standard Illumina Read 1 Sequencing primer. A primer mixture would result in low clusters calls and the resulting reads would be contaminated by poly(T) stretches.