The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) is compatible with the following kits and modules:
QuantSeq 3' mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, 113 – 115, 129 – 131)
QuantSeq-Flex Targeted RNA-Seq Library Prep Kit V2 with First Strand Synthesis Module (Cat. No. 033) using oligo(dT) or target-specific first strand synthesis priming and random primed second strand synthesis
QuantSeq-Flex First Strand Synthesis Module for Illumina (Cat. No. 026)
Globin Block Modules for QuantSeq (Human and Pig, Cat. No. 070, 071)
i5 Dual Indexing Add-on Kits for QuantSeq / CORALL (Cat. No. 047)
PCR Add-on Kit for Illumina (Cat. No. 020)
This UMI module is not compatible with the following kits:
QuantSeq 3' mRNA-Seq Library Prep Kit REV (for Illumina, Cat. No. 016)
QuantSeq-Flex First Strand Synthesis Module of Illumina V2 (Cat. No. 166)
QuantSeq-Flex Second Strand Synthesis Module for Illumina (Cat. No. 028)
CORALL mRNA-Seq and CORALL Total RNA-Seq Library Prep Kits (Cat. No. 158 – 163 for CORALL mRNA-Seq and Cat. No. 095, 096, 117 – 119, 132 – 134, 146, 147 for CORALL Total RNA-Seq)
Small RNA-Seq Library Prep Kit for Illumina (Cat. No. 052, 058, 135)
The UMI Second Strand Synthesis Module for QuantSeq is also not compatible with, and not recommended for use with, any other external library prep protocols or kits.
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