Typically, libraries prepared from blood total RNA using Globin Block require one additional PCR cycle compared to libraries made with the standard QuantSeq Kit reagents.
However, we strongly recommend performing a qPCR assay to determine the optimal number of PCR cycles for the library amplification. The PCR Add-on Kit for Illumina (Cat. No. 020.96) includes PCR and Enzyme Mix reagents and P7 primer (7000) that are required for the qPCR assay. In addition, SYBR Green I Nucleic Acid Gel Stain (10,000X in DMSO, Sigma-Aldrich Cat. No. S9430) is also required (user provided).
Further details of the qPCR assay are provided in these FAQs:
What do I need for the qPCR Assay?
How do I calculate the Endpoint PCR cycle number from the qPCR assay?