Lexogen Online FAQs
What recommendations do you have for targeting rare, or low-abundance transcripts?
Maximize the input amounts of RNA where possible.
Use 1 hour incubation time for reverse transcription (step 4).
Use 48 µl of Purification Solution (PS) at step 16.
Perform a qPCR assay to determine the optimal cycle number for library amplification.
See these FAQs for further details:
What do I need for the qPCR Assay?
How do I calculate endpoint cycle numbers?
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