Lexogen Online FAQs

What is the effect of gDNA contamination on my data?

Owned by Simona Ferraioli (Unlicensed)
Last updated: May 09, 2022
1 min read

The presence of gDNA contamination can strongly impact the sequencing data and lead to biases and quantification issues during data analysis.
A typical signature of gDNA contamination includes:

  • A high proportion of intronic and intergenic reads

  • Loss of strand specificity (i.e., reads mapping to both strands)

 

NOTE: for more information on gDNA contamination and DNAse treatment, please, check our RNA LEXICON Chapter #5.

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