The kit includes a set of 96 i1 indices (i1 12 nt RT-Set for QuantSeq-Pool, dried-in sample barcodes in 96-well plate format), which allow up to 96 samples to be sequenced per lane on an Illumina flow cell.
For multiplexing of more than 96 samples, unique dual indexing for the individual pools (containing up to 96 sample-barcoded libraries) is recommended.
Lexogen offers various indexing systems that are fully compatible. We recommend using Lexogen's 12nt Unique Dual Indexing System with QuantSeq-Pool experiments. Unique Dual Indices (UDIs) with pre-mixed i5 and i7 indices are available in a convenient 96-well format. UDIs are 12 nucleotides in length and provide superior error correction capability for massive multiplexing. The Lexogen UDI 12 nt Unique Dual Indexing Sets are available in sets of 96 (Cat. No. 101 – 105) for multiplexing of up to 9,216 samples per set, or 36,864 samples with just 384 UDIs (Cat. No. 156).
Lexogen also offers 6 nt single indexing sets for i7 (Cat. No. 044.96) and i5 (Cat. No. 047.96). These 6 nt Index Sets can be used separately as single indices for either i5 or i7, or can be combined for dual indexing.
Lexogen Online FAQs
Manage space
Manage content
Integrations