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The PCR Add-on kit contains a PCR Mix including a non-indexed Illumina P5 specific primer, a thermostable polymerase, and an Illumina P7 primer without a barcode (primer 7000). In addition, a reamplification primer for single-indexed (i7 only ) is included. The kit can be used for two purposes:

  • The qPCR Assay

  • Reamplification of single-indexed QuantSeq and CORALL libraries

NOTE: This kit is not compatible with the small RNA-Seq Library Prep Kit.

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