Processing high quality RNA from bacterial monocultures with the Gram Positive or Gram Negative Probe Mixes typically results in less than 1 % remaining rRNA reads. Depletion rates for the META Probe Mix using RNA from mixed bacterial samples can vary depending on the sample. Depletion rates may vary depending on the quality of the RNA and purity of the sample. Contaminants, such as salts, metal ions, and organic solvents, may have a negative impact on the efficiency of the protocol.
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