Lexogen Online FAQs

Do I need an on-deck thermocycler?

This option is up to the user. Using an on-deck thermocycler provides a higher level of automation compared to an off-deck thermocycler. However, both options will require manual interventions. The use of on-deck thermocyclers may not be possible with all instrument types, and this must also be specified in the automation script protocol.

If a stand-alone thermocycler is available in a comfortable vicinity of the robot, we can suggest to use an off-deck thermocycler for steps 2 – 3, and step 4 of the QuantSeq protocol, as conventional thermocyclers provide heated lid options that prevent condensation. Depending on the type of on-deck thermocycler used, a heated lid option may not be provided.


The current Perkin Elmer Sciclone, Eppendorf EpMotion 5075, Hamilton STAR / STARlet / NGS STAR, and Agilent Bravo Workstation (B) protocols are setup for off-deck thermocyclers.

The current Beckman Coulter Biomek FxP / i7 protocols are written for on-deck thermocycling at all steps, including the PCR. However, modifications to all protocols can be made in collaboration with the respective liquid handler manufacturer.


Have feedback for us or need more information? Send us a request or write to us at support@lexogen.com.