Working Copy: What is the difference between LUTHOR 3’ mRNA-Seq and QuantSeq 3’ mRNA-Seq?

LUTHOR utilizes a pre-amplification step.
LUTHOR libraries are therefore generated from amplified RNA molecules.
The pre-amplification step enables the generation of libraries from ultra-low input RNA, down to the single-cell level.

RNA amplification in LUTHOR is templated by the endogenous mRNA molecules instead of using cDNA intermediates.

QuantSeq utilizes a different chemistry where no pre-amplification step is employed, requiring higher RNA input amounts.

QuantSeq captures the 3' end of transcripts by oligo(dT) priming and reverse transcription.
During reverse transcription, a partial Illumina compatible adapter is introduced at the 3' end. After removal of the template RNA, the second strand is generated by random priming and extension using a DNA polymerase.