Which 6nt i5 Dual Indexing Add-on Kit do I need?

The i5 6 nt Unique Dual Indexing Add-on Kit (Cat. No. 047.96) provides a 96-well plate containing 96 unique i5 indices . This kit is designed for unique dual indexing in combination with Lexogen’s 6 nt i7 indices included in the standard QuantSeq FWD/REV kits (Cat. No. 015 and 016). Up to 96 uniquely dual-indexed libraries can be prepared for sequencing in a single lane or run.

The i5 6 nt Dual Indexing Add-on Kit ( Cat. No. 047.4x96) provides four different i5 index primers (5001 – 5004). Each tube contains sufficient volume for preparing 96 libraries. This kit is therefore ideal for combinatorial dual indexing strategies (see What is the advantage of unique dual indexing versus combinatorial dual indexing?archived). In combination with 96 6 nt i7 indices a maximum of 384 (4 i5 x 96 i7) dual-indexed libraries with unique i5 / i7 index combinations can be multiplexed in a single sequencing lane or run.

Alternatively, up to 384 pre-mixed UDIs of the Lexogen 12 nt Unique Dual Indexing Add-on Kits (Cat. No. 107-111.96 and 120.384) can be used to replace the i7 6 nt indices provided in the standard QuantSeq 3’ mRNA-Seq FWD/REV, and CORALL RNA-Seq Library Prep Kits.