Lexogen Online FAQs
What are the differences between QS FFPE and QuantSeq V2 kits?
The reagents for library generation and the reaction volumes differ between QuantSeq FFPE and QuantSeq V2 protocols. Specifically:
| QuantSeq FFPE | QuantSeq V2* |
First strand cDNA synthesis |
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RNA removal |
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Second Strand Synthesis |
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Purification |
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The overall protocol time remains similar between the two library preps, with a total prep time of 4.5 hours (1.45 hours of hands-on time).
* Reaction components and volumes are provided according to the QuantSeq V2 protocol (with modifications for FFPE RNA samples).
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