The QuantSeq 3‘ mRNA-Seq V2 Library Prep Kit REV with Unique Dual Indices (12nt, Cat. No. 225) replaces the previous version of the QuantSeq 3‘ mRNA-Seq Library Prep Kit REV (Cat. No. 116).
In the V2 kits, there are several component changes:
First Stand cDNA Synthesis Mix “FS1” and First Strand cDNA Synthesis Mix 2 “FS2” are replaced with 10x First Strand cDNA Synthesis Mix “10xFS”, OligodT primer REV “dT REV”, and “DTT”
PCR Mix “PCR” and Enzyme Mix 3 “E3” are replaced with PCR Mix “PM” and PCR Enzyme Mix “PE”
Lexogen i7 6nt Index Set is replaced with Lexogen UCI 12nt Set
The QuantSeq-REV V2 Kits allows for lower RNA input amounts, down to 1 ng to total RNA. A modified protocol, included in the QuantSeq-REV V2 User Guide, can be used for such low inputs. Finally, higher RNA input volumes, up to 16 μl, can be used.