The LUTHOR single cell dispenser uses impedance changes to detect and record the passage of cells through the LUTHOR scd Tip aperture. When a particle (e.g., single cell) passes through the aperture of the tip, an electrical signature is detected and translated as a unique peak on the electrogram.At the end of dispensing, the LUTHOR single cell dispenser’s analysis software will perform a quality control and indicate which wells pass (tagged in green) and which wells fail (tagged in red).
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Figure | Electric signal of a single cell (Green arrow) versus particles (Red arrows).
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NOTE: The presence of multiple peaks results from the detection of multiple cells and/or aggregates (see FAQ: What does the impedance signal of a single cells look like? What about a doublet?)
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