How does the LUTHOR single cell dispenser work?

The LUTHOR single cell dispenser uses impedance changes to detect and record the passage of cells through the LUTHOR scd Tip aperture. When a particle (e.g., single cell) passes through the aperture of the tip, an electrical signature is detected and translated as a unique peak on the electrogram. At the end of dispensing, the LUTHOR single cell dispenser’s analysis software will perform a quality control and indicate which wells pass (tagged in green) and which wells fail (tagged in red).

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NOTE: The presence of multiple peaks results from the detection of multiple cells and/or aggregates (see FAQ: What does the impedance signal of a single cells look like? What about a doublet?)