The Small RNA-Seq kit can be used for inputs from 100 ng – 1,000 ng of cellular total RNA or 50 pg – 1,000 ng of enriched small RNA including low RNA content samples such as plasma, serum, and urine.
In general, we recommend using 100 ng of total RNA input from cells or tissues.
For 100 ng input amounts, we recommend using 0.5x dilution of 3' Adapter, 5' Adapter, and RT-Primer and 50 µl of EtOH.
Input amounts lower than 100 ng require further adapter dilutions and protocol adjustments. For further information, see this FAQ and Appendix B of the Small RNA-Seq User Guide (052UG128).