What are the input RNA requirements?

The Small RNA-Seq kit can be used for inputs from:

• 100 ng – 1,000 ng of cellular total RNA or whole blood RNA;

• 50 pg – 1,000 ng of enriched small RNA and low RNA content samples such as plasma, serum, urine, exosomal RNA, etc.

In general, we recommend using >100 ng of total RNA input from cells or tissues.

For 1 - 100 ng input amounts, we recommend using 0.5x dilution of 3' Adapter (A3), 5' Adapter (A5) and Reverse Transcription Primer (RTP) and 50 µl of EtOH in Step 5 of the library prep protocol.

Input amounts lower than 1 ng require further adapter dilutions and protocol adjustments. For more information, see this FAQ and Appendix B of the Small RNA-Seq User Guide.