No. QuantSeq-Pool requires asymmetric paired-end sequencing, where Read 1 is generated towards the poly(A) tail and directly corresponds to the mRNA sequence. Read 2 will contain the Unique Molecular Identifier (UMI) followed by the i1 sample-barcode. Therefore, it is necessary to sequence Read 2 for sample identification, i1 barcode demultiplexing and UMI deduplication.
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