Lexogen Online FAQs
Can I use single-read sequencing for QuantSeq-Pool libraries?
- Matteo Carina (Unlicensed)
- Stephanie Bannister
Owned by Matteo Carina (Unlicensed)
No. QuantSeq-Pool requires asymmetric, paired-end sequencing. It is necessary to sequence Read 2 for sample identification, i1 barcode demultiplexing and UMI deduplication.
Read 1 is generated towards the poly(A) tail and directly corresponds to the mRNA sequence. This read will be trimmed and mapped to report transcript expression levels.
Read 2 will contain the Unique Molecular Identifier (UMI) followed by the i1 sample-barcode. This read is required for demultiplexing and read de-duplication following alignment.
See: What read length should I use for QuantSeq-Pool Reads 1 and 2? for recommended read lengths.
Have feedback for us or need more information? Send us a request or write to us at support@lexogen.com.