No. QuantSeq-Pool requires asymmetric, paired-end sequencing, where . It is necessary to sequence Read 2 for sample identification, i1 barcode demultiplexing and UMI deduplication.
Read 1 is generated towards the poly(A) tail and directly corresponds to the mRNA sequence. This read will be trimmed and mapped to report transcript expression levels.
Read 2 will contain the Unique Molecular Identifier (UMI) followed by the i1 sample-barcode. Therefore, it is necessary to sequence Read 2 for sample identification, i1 barcode demultiplexing and UMI deduplicationThis read is required for demultiplexing and read de-duplication following alignment.
See: What read length should I use for QuantSeq-Pool Reads 1 and 2? for recommended read lengths.