The i5 6 nt Unique Dual Indexing Add-on Kit (Cat. No. 047.96) provides a 96-well plate containing 96 unique i5 indices (Cat. No. 047.96) . This kit is designed for unique dual indexing in combination with Lexogen’s 6 nt i7 indices ( included in the standard QuantSeq 3’ REV and Flex library prep FWD/REV kits (Cat. No. 016, 033, 034,and035015 and 016). Up to 96 uniquely dual-indexed libraries can be prepared for sequencing in a single lane or run.
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The i5 6 nt Dual Indexing Add-on Kit ( Cat. No. 047.4x96) provides four different i5 index primers (5001 – 5004). Each tube contains sufficient volume for preparing 96 libraries. This kit is therefore ideal for combinatorial dual indexing strategies (see [FAQ 4.6] What is the advantage of unique dual indexing versus combinatorial dual indexing?). In combination with 96 6 nt i7 indices a maximum of 384 (4 i5 x 96 i7) dual-indexed libraries with unique i5 / i7 index combinations can be multiplexed in a single sequencing lane or run.
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