The i5 6 nt Unique Dual Indexing Add-on Kit provides a 96-well plate containing 96 unique i5 indices (Cat. No. 047.96). This kit is designed for unique dual indexing in combination with Lexogen’s 6 nt i7 indices (included in the QuantSeq 3’ REV and Flex library prep kits (Cat. No. 016, 033, 034,and 035). Up to 96 uniquely dual-indexed libraries can be prepared for sequencing in a single lane or run.
The i5 6 nt Dual Indexing Add-on ( Cat. No. 047.4x96) provides four different i5 index primers (5001 – 5004). Each tube contains sufficient volume for preparing 96 libraries. This kit is therefore ideal for combinatorial dual indexing strategies (see [FAQ 4.6]). In combination with 96 6 nt i7 indices a maximum of 384 (4 i5 x 96 i7) dual-indexed libraries with unique i5 / i7 index combinations can be multiplexed in a single sequencing lane or run.
Alternatively, up to 384 pre-mixed UDIs of the Lexogen 12 nt Unique Dual Indexing Add-on Kits (Cat. No. 107-111.96 and 120.384) can be used to replace the i7 6 nt indices provided in the standard QuantSeq 3’ mRNA-Seq FWD/REV, and CORALL RNA-Seq Library Prep Kits.