No, it depends on what kind of targeted sequencing you intend to do. The modules are compatible with the QuantSeq FWD kits (Cat. No. 015, 113 – 115, 129 – 131). By getting
Using only the QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166), you can generate target-specifically primed specific libraries during using customized reverse transcription and use the random primers, with random-primed Second Strand Synthesis from QuantSeq, generating . This generates targeted RNA-Seq libraries, allowing the identification of novel fusions.
By purchasing
Using only the QuantSeq-Flex Second Strand Synthesis Module (Cat. No. 028), you can generate reverse transcription uses oligo(dT) primed libraries during reverse transcription (using the First Strand Synthesis of QuantSeq) priming and target-specifically primed libraries during specific primers are used for second strand synthesis, which means . This generates targeted 3' mRNA-Seq libraries. Please note that the
Using both the QuantSeq-Flex First and Second Strand Synthesis Modules allows you to generate fully-targeted libraries to identify known targets at specified sequence regions. This is the ideal approach when seeking to replace multiplex qPCR with an Amplicon-based targeted RNA-Seq assay.
NOTE: The second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need to use lower temperatures, please contact support@lexogen.com.
Getting both the QuantSeq-Flex First and Second Strand Synthesis Modules allows you to generate targeted libraries in both reverse transcription and second strand synthesis (targeted RNA-Seq) to identify known targets.