No, it depends on what kind of targeted sequencing you intend to do. Both modules are compatible with the QuantSeq FWD V2 (Cat. No. 191 - 196).
Using only the QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166), you can generate target-specific libraries using customized reverse transcription primers, with random-primed second strand synthesis. This generates targeted RNA-Seq libraries, allowing the identification of novel fusions.
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Using both the QuantSeq-Flex First and Second Strand Synthesis Modules allows you to generate fully-targeted libraries to identify known targets at specified sequence regions. This is the ideal approach when seeking to replace multiplex qPCR with an Aampliconamplicon-based targeted RNA-Seq assay.
NOTE: The second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need to use lower temperatures, please contact support@lexogen.com.
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