Yes, the i5 Provided that the recommended partial adapter sequences are included on target-specific primers, Lexogen’s 12 nt Unique Dual Indexing Add-on Kits (UDIs) or i5 6 nt Dual Indexing Add-on Kits can be used for dual indexing of QuantSeq-Flex libraries. When designing targeted primers for QuantSeq-Flex, the partial P5 and P7 adapter sequences need to be included to enable library amplification using the i5 and i7 index primers. Please refer to the i5 Dual Indexing Add-on Kits Instruction Manual (047IM109) for details of dual-indexed library amplification and purification.
For further information about the
See these FAQs for further information on dual indexing strategies:
Which 6nt i5 Dual Indexing Add-on Kit for QuantSeq, please see the online Frequently Asked Questions (FAQs) on the QuantSeq FWD product page. do I need?
12 nt Unique Dual Indexing Solutions