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RTL is designed to generate longer inserts and should be used with intact input RNA. If RTL is used with degraded / FFPE RNA, library length may be shortened, yield may be impacted, and more artifacts may be generated.
Our new CORALL FFPE Whole Transcriptome RNA-Seq library prep (Cat. No. 219-220, 233-234) has been specifically optimized for processing FFPE samples.