Either 24 reactions isolating large RNAs plus 24 reactions isolating small RNAs or, alternatively, 48 reactions isolating only the large RNAs or total RNA can be performedSPLIT contains enough reagents to extract total RNA from 48 samples.
If desired, a supplementary protocol is available for the extraction of large and small RNA-enriched fractions. With this workflow, SPLIT contains enough reagents for the isolation of the large RNA-enriched fraction from 48 samples, or small and large RNA-enriched fractions from 24 samples. Please see Appendix D in the User Guide for more information.