No, it depends on what kind of targeted sequencing you intend to do. The Both modules are compatible with the QuantSeq with UDI FWD V2 kits (Cat. No. 191 - 196) and with the QuantSeq FWD V1 kits (Cat. No. 015, 113 – 115, 129 – 131).
Using only the QuantSeq-Flex First Strand Synthesis Module V2 (Cat. No. 166), you can generate target-specific libraries using customized reverse transcription primers, with random-primed Second Strand Synthesissecond strand synthesis. This generates targeted RNA-Seq libraries, allowing the identification of novel fusions.
Using only the QuantSeq-Flex Second Strand Synthesis Module (Cat. No. 028), reverse transcription uses oligo(dT) priming and target-specific primers are used for second strand synthesis. This generates targeted 3' mRNA-Seq libraries.
NOTE: If interested in using QuantSeq-Flex Second Strand Synthesis Module with QuantSeq REV, please contact support@lexogen.com.
Using both the QuantSeq-Flex First and Second Strand Synthesis Modules allows you to generate fully-targeted libraries to identify known targets at specified sequence regions. This is the ideal approach when seeking to replace multiplex qPCR with an Ampliconamplicon-based targeted RNA-Seq assay.
NOTE: The second strand synthesis reaction requires annealing temperatures of >45 °C. In case you need to use lower temperatures, please contact support@lexogen.com.