The Small RNA-Seq kit can be used for inputs from:
100 ng – 1,000 ng of cellular total RNA, enriched small RNA or whole blood RNA;
50 pg –
...
100 ng of
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low RNA content samples such as plasma, serum,
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urine, exosomal RNA, etc.
In general, we recommend using 100 ng of total RNA input from cells or tissues.
For 1 -100 ng input amounts, we recommend using 0.5x dilution of 3' Adapter (A3), 5' Adapter
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(A5) and Reverse Transcription Primer (RTP) and 50 µl of EtOH in Step 5 of the library prep protocol.
Input amounts lower than
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1 ng require further adapter dilutions and protocol adjustments. For
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more information, see this FAQ and Appendix B of the Small RNA-Seq User Guide
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.