The Small RNA-Seq kit can be used for inputs from:
...
For 1 -100 ng input amounts, we recommend using 0.5x dilution of 3' Adapter (A3), 5' Adapter (A5) and Reverse Transcription Primer (RTP) , and 50 µl of EtOH in Step 5 of the library prep protocol.
Input amounts lower than 1 ng require further adapter dilutions and protocol adjustments. For more information, see this FAQ and Appendix B of the Small RNA-Seq User Guide.