To achieve a higher nucleic acid concentration, the amount of Lysis Buffer can be reduced from 110 μL to a minimum of 70 μL. This is beneficial for samples with a small total surface area. However, the reduction my result in a more difficult handling and a lower yield, especially if the FFPE sample contains excess paraffin.
If the volume of Lysis Buffer is reduced, please ensure that at least 50 μL is applied to the Spin Column.