Cells should be in a single-cell suspension in any conductive cell culture medium or buffer.
The ideal input is a minimum of 150,000 cells in at least 1ml cell culture medium or buffer.
1ml is the minimal volume required to use with the cell strainer.
IMPORTANT: When using lower volumes (i.e., <1ml), it is not possible to use the cell strainer to filter the cell suspension. In this case, please contact technical support at support@lexogen.com for further recommendations.
Once the cells are filtered, please adjust the cell concentration to 1.5.10^5 cells/ml. Then 15µl will be used for the sample preparation with the LUTHOR scd buffer for a final working concentration of 15cells/µl (15.000 cells/ml).
IMPORTANT: Lower cell numbers can also be used (down to 100 cells). Please contact technical support at support@lexogen.com for further information.
NOTE: If cells tend to aggregate, it is possible to optimize the medium using serum-free, Ca2+/Mg2+- free medium, or by adding chelators, DNase, or anti-clumping compounds.