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When using the BC1 Block Module with low quality RNA, we recommend the following modifications to the QuantSeq protocol:

  • Skipping step 2

  • Reduce the amount of PS added in step 16 (48 μl instead of 56 μl)

  • Reduce the amount of PB in step 29 (31.5 μl PB instead of 35 μl PB for dual indexed libraries and 27 μl PB instead of 30 μl PB for single indexed libraries, respectively).

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